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May 23, 2019 | 11:00 a.m. - 12:00 p.m.
Category: Seminar
Location: Scott Hall #3125 | Map
540 E. Canfield
Detroit, MI 48201
Cost: Free
Audience: Current Graduate Students, Faculty

Jeanne Stachowiak, PhD
Associate Professor, Banks McLaurin Faculty Fellow, Department of Biomedical Engineering, Institute for Cellular and Molecular Biology, University of Texas at Austin

“Stochastic Mechanisms in Membrane Traffic”

Abstract

Membrane traffic, an essential cellular process that plays a role in many human diseases, requires key biophysical steps including formation of membrane buds, loading of these buds with specific molecular cargo, and separation from the parent membrane through the process of membrane fission. The prevailing view has been that structured protein motifs such as wedge-like amphipathic helices, crescent-shaped BAR domains, curved coats, and constricting dynamin rings drive these processes. However, many proteins that contain these structural motifs also contain large intrinsically disordered protein (IDP) domains of 300-1500 amino acids, including most clathrin and COPII coat components. While these IDP domains have been regarded primarily as flexible biochemical scaffolds, we have recently discovered that IDPs are highly efficient physical drivers of membrane budding fission. How can molecules without a defined structure drive membrane remodeling? Our results support the idea that disordered domains generate entropic pressure at membrane surfaces, which is critical to overcoming key biophysical barriers to membrane traffic. IDPs are particularly efficient generators of entropic pressure owing to their very large hydrodynamic radii, potential for electrostatic repulsion owing to high net charge, and the substantial entropic cost of extending them. More broadly our findings suggest that any protein, regardless of structure, can contribute to membrane remodeling by increasing entropic pressure, and paradoxically, that proteins that lack a defined secondary structure, IDPs, may be among the most potent drivers of membrane fission.

For more information about this event, please contact Suzanne Shaw at 5775325 or sshaw@wayne.edu.